Business: Overview

 

Nanomorphomics in health and disease at St Andrews in the Lucocq lab - Quantitative nanoscale imaging and molecule mapping using electron microscopy

 

Nanoparticle imaging and analysis

Nanodiscs, bicelles, exosomes, liposomes, protein complexes, multifunctional nanoparticles.

Uses novel and rapid electron imaging and characterisation by localising lipids, proteins or chemical groups to the particles; measurement of nanoparticle concentration.

 

Quantitative molecular mapping on intact cells or organelles

Proteins, signaling lipid and lipid droplets in normal and pathological specimens.

Uses digital readout gold labelling quantified using rapid unbiased quantitation on thawed frozen sections and freeze substitution technology combined with novel lipid retaining methods. New approaches ensure readouts are real/specific distributions of proteins/lipids by removing background.

Expression profiling at the protein and transcript level now possible.

 

Widescale quantitative imaging of intact cells/organelles (stem cells, tissues, bacteria, parasites, tumours)

Quantification of volume, surface, number, length (stereological analysis).

Serial section analysis and 3D reconstructions.

Spatial mapping analysis in 3D.

 

Nanoparticle localisation and quantitative analysis and spatial mapping in intact cells

Rapid quantification of nanoparticle distributions over a range of organelles/compartments.

Target compartment identification using double label approaches.

Secondary characterisation of nanoparticle composition in situ.

Spatial mapping and quantitation of nanoparticles both within whole organisms and within cells.